Pulse perturbation technique for determination of piroxicam in pharmaceuticals using an oscillatory reaction system

Abstract

A simple and reliable novel kinetic method for the determination of piroxicam (PX) was proposed and validated. For quantitative determination of PX, the Bray-Liebhafsky (BL) oscillatory reaction was used in a stable non-equilibrium stationary state close to the bifurcation point. Under the optimized reaction conditions (T = 55.0 C, [H2SO4] 0 = 7.60×10-2 mol L-1, [KIO 3]0 = 5.90×10-2 mol L-1, [H2O2]0 = 1.50×10-1 mol L -1 and j 0 = 2.95×10-2 min-1), the linear relationship between maximal potential shift ΔE m, and PX concentration was obtained in the concentration range 11.2-480.5 μg mL-1 with a detection limit of 9.9 μg mL-1. The method had a rather good sample throughput of 25 samples h-1 with a precision RSD = 4.7% as well as recoveries RCV ≤ 104.4%. Applicability of the proposed method to the direct determination of piroxicam in different pharmaceutical formulations (tablets, ampoules and gel) was demonstrated. © 2013 Versita Warsaw and Springer-Verlag Wien.